Thanks Simon,

I ran it with method="blind" and fitType="local" and that did the trick.

Thanks so much for your help.

Alex


On Mon, Jul 15, 2013 at 2:20 PM, Simon Anders <anders@embl.de> wrote:

> Hi Alex
>
>
> On 15/07/13 19:33, Alex Koeppel wrote:
>
>> However, when I do estimateDispersions(method="**blind") on the same
>> input, I
>> get the following error:
>>
>> "Error in parametricDispersionFit(means, disps) :
>>    Parametric dispersion fit failed. Try a local fit and/or a pooled
>> estimation. (See '?estimateDispersions')"
>>
>> I tried using method="pooled", as the message recommends, and that did
>> work, but I'm not completely sure if it is ok to use the pooled results
>> for
>> my PCA or not.
>>
>
> No, pooled estimation is not appropriate for PCA. You should rather try a
> local fit (fitType="local").
>
>   Simon
>
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