Hi-
   In the roast paper, the examples tended to have extra samples not used to define the contrast - which helped increase the degrees of freedom for the rotation test (e.g. 26 samples in a 3 group experiment; group 1 has 3 samples, group 2 has 3 samples and group 3 has 20 samples. - the contrast compared group 1 and group 2).   Is running roast on a dataset with 6 samples in 2 groups (3 test, 3 control) an acceptable use case or will the degrees of freedom be too small to get valid p-values?

Thanks,
Julie

Julie Leonard
Computational Biologist
Global Bioinformatics

Syngenta Biotechnology, Inc.
3054 E. Cornwallis Rd.
Research Triangle Park, NC 27709
USA


julie.leonard@syngenta.com<mailto:julie.leonard@syngenta.com>
www.syngenta.com<http://www.syngenta.com/>




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