Hi Yongzi,

The file you attached doesn't seems to be a valid. Please use raw files
from the GEO page.

Also, you might want to put your code in mail.


Atul



On 06/16/2013 09:31 PM, yongzi chen wrote:
> To whom it may concern.
>
> Hello, I am new in microarray data analysis. recently I want to use Limma to find different expressed genes between grade I and grade 
> III, which you could see from the annotation file. but when I use the R 
> code below to do this, I only got two different expressed genes, could 
> anybody tell me why? Is there anything wrong with my code? thank you in 
> advance! and the data is available on line.http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE2990.
>  
>
> Best regards,
>
> yongzi
>
>
>
> Best wishes to you!
>
>
> ======================================================
> Yong-Zi Chen, Ph.D.
>  
> Tianjin Cancer Institute
> Tianjin Medical University Cancer Institute and Hospital
> Huan-Hu-Xi Road, Ti-Yuan-Bei, He-Xi District
> Tianjin300060, P.R. China
> Phone&Fax:   0086-022-23340123
>
>
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