We are running experiments where there are technical difficulties in getting a pure template of interest.  What we have is a mix of template-1 (our template of interest) and template-2 (the contaminating template).  We have pure samples of template-2 that we run alongside the mix.  We also run several HSK genes to use for normalization.

Using HTqPCR, is there a way to remove the signal in the mix due to template-2 ?

Thanks.

Susanne


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