Dear sir,

               I am doing differential expression analysis with limma. In
ebFit = eBayes(fit2) I am getting error:  Error in ebayes(fit = fit,
proportion = proportion, stdev.coef.lim = stdev.coef.lim,  :  No residual
degrees of freedom in linear model fits. It is may be because I don't have
replicates. So what should I do to for differential expression now.

 

 

Thank you

Nitin Mandloi

 

Sandor Proteomics Pvt. Ltd., hyderabad


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