Hi all,

I'm quite new to flow cytometry, since i have mostly perfomed
transcritionnal analyses before. I discovered the flowCore, flowViz,
flowXXX packages in bioconductor and found it amazing.

I have a series of data with two different panels (tubes) for each patient.
As there are some common markers in both panels, i was wondering if i could
merge the two into a single flowSet with more channels.

In details :

   - for panel A I have SSC, FSC, CD45, CD3, CD4, CD8, HLA-DR
   - for panel B I have SSC, FSC, CD45, CD3, CD5, CD19, CD16

I would like to obtain a single merged-panel with : SSC, FSC, CD3, CD45,
CD4, CD8, CD5, CD19, CD16, HLA-DR

I was thinking that the dataset is composed roughly of a matrix with for
each leukocyte (event, =row) a value for all channels above (columns). So,
maybe it would be possible to try to clusterise by pairs the rows of panel
A and those of panel B on common values of SSC, FSC, CD45 and CD3. The
result dataset would take the mean of the common channels and then the
single values for CD4, CD5, CD8, CD16, CD19, HLA-DR.

I would be glad to have your comment on this before starting. Maybe it has
already been done in some package, or maybe one would have a comment to
take into accoutn before i start ?

Thanks in advance.
Julien

-- 
Envoyé de mon ENIAC

Julien Textoris, MD, PhD
Laboratoire d'immunologie,
UMR CNRS 7278, INSERM U1095
Faculté de Médecine Timone, Marseille
+33 (0)4 91 32 49 71

Service d'anesthésie et de réanimation
Hôpital Nord, Marseille
+33 (0)4 91 96 55 31

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