Hi all!, 

We are trying to run cellHTS2 sample dataset for dual channel screen and it ended up with following error message: 

Error in summarizeReplicates(xsc, summary = "mean") : 
  Currently this function is implemented only for single-color data.

>From the FAQ it seems like this is pointing to the step 2 but this is not clear for us. 

http://rnai-screening-wiki.dkfz.de/signaling/wiki/display/rnaiwiki/web+cellHTS2+Manual
Currently this function is implemented only for single-color data. 
Channel 2 was labeld to one of your files in step 

The pseudo code we were trying  as follows: 

>>library("cellHTS2")
>>expName <- "DualChannelScreen"
>>datadir <- system.file("DualChannelScreen", package="cellHTS2")
>>x <- readPlateList("Platelist.txt", name=expName, path=datadir)
>>x <- configure(x, "Description.txt", "Plateconf.txt", "Screenlog.txt", path=datadir)
>>xn = normalizePlates(x, scale="multiplicative", log=FALSE, method="median", varianceAdjust="none")
>>xsc = scoreReplicates(xn, sign="-", method="zscore")
>>xsc = summarizeReplicates(xsc, summary="mean")
>>xsc = annotate(xsc, geneIDFile="GeneIDs.txt", path=datadir)

Hope someone can give more insight on this error message. 

Many thanks in advance, 
Vipin


	[[alternative HTML version deleted]]

