HI all,

I have paired-end RNAdata from five different tissues of the same organism,
I calculated the read counts using HTSeq tools and now I want to see which
are the transcripts which are differentially expressed (if any) among the
five tissues (I do not have any replicates). I have tried using edgeR and
DESeq packages but not getting even a single value to be significant. Can
anyone suggest the possibilities??


Regards,
Srikanth

-- 
Srinivas M. Srikanth
Ph.D. Student
Institute of Bioinformatics
Discoverer, 7th Floor,
International Technology Park,
Bangalore, India
Mob:+919019114878

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