hi all,
i am trying to normalize ,segment and process  gene expression data using r
version 2.15.1
iam stuck at nomalization where in i have tried the following commands.


>library(marray)

>library(convert)

>folder <- 'C:/man/'

>file <- sort (dir(folder))

>o <- order(as.numeric(sub( 'OC ', '', sub('.txt', '', file))))

>file <- file[o]

>data <- read.Agilent(fnames=file)

>p <- order(data@maGnames@maInfo$ProbeName)

>data <- data[p,]

>normData <- maNorm(data)

>normData@maLayout@maSub <- TRUE

>normData@maLayout@maNspots <- nrow(normData@maM)

>ma <- as(normData, 'MAList')

>source('readPositionalInfo.R')       # this command it returns me an error

Error in file(filename, "r", encoding = encoding) :
  cannot open the connection
In addition: Warning message:
In file(filename, "r", encoding = encoding) :
  cannot open file 'readPositionalInfo.R': No such file or directory



i have even tried making a clone info file using this format in an excel
sheet and saved it as (cloneinfo.txt) but this is also not being accepted.

probe_id     Chr     Position


could anyone help me with as to how to proceed next and can we use any
other command instead of source('readPositionalInfo.R').

any help will be greatly appreciated
thanks
adesh

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