Dear Simon, Thanks a lot for your response. ----- Forwarded Message ----- From: Simon Anders To: bioconductor@r-project.org Sent: Wednesday, 8 August 2012 6:55 PM Subject: Re: [BioC] deseq within and between group variation Dear Sudeep On 2012-08-07 18:31, sudeep s wrote: > Is there a way to calculate within and between group variability > between two sample groups (with biological replicates) within DESeq ? > I went through DESeq manual, but couldn't find any reference.  So is > the only way is to calculate it directly from the normalized read > count data ? Count data is not homoscedastic and hence, there is no point in looking at within and between group variance or using other methods from standard linear regression methods. What you want is within and between group _deviance_, and these are calculated by the 'fitNbinomGLMs' function. Please read up in on generalized linear models (GLMs) and analysis of deviance (ANODEV).   Simon _______________________________________________ Bioconductor mailing list Bioconductor@r-project.org https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor [[alternative HTML version deleted]]