Hi Carmen,

A few questions to get a better idea of the experiment.

Firstly, what do you mean by d norm? delta Cq method? I.e. subtraction of
reference genes?

How many samples do you have? How many conditions? and how many genes in
each samples?

You say you have different RNA input in every sample, what do you mean by
this? Do you have biological replication?

Cheers,

Jim

On 25 July 2012 23:09, Carmen Palacios <cyapalacios@gmail.com> wrote:

> Hi all,
>
> I use qPCR array of 384 wells and I normalize using d.norm.
> But I used different RNA input in every sample, so I thinks the
> differentially expressed genes are not right.
> I think I need to normalize in another way  before d.norm, but I don't know
> how to do it.
> How may I found the more accurate method?
>
> Thank you in advance.
>
> Atte, Carmen Palacios.
>
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