Dear all,

I would appreciate a suggestion about edgeR : I have a GRO-seq dataset (CTL
vs TREAT, no replicates, just 1 sample for CTL and 1 sample for TREAT
condition),
and I am assessing the differential expression by using edgeR, but I am
getting an error : any suggestion would be appreciated. thanks very much !

> d <- estimateCommonDisp(d)
Warning message:
In estimateCommonDisp(d) :
  There is no replication, setting dispersion to NA.

> de.com <- exactTest(d)
Error in if (any(dispersion < 0)) stop("dispersion must be non-negative") :
  missing value where TRUE/FALSE needed

> de.com <- exactTest(d,dispersion=0)
Error in binomTest(s1[pois], s2[pois], p = n1/(n1 + n2)) :
  y1 and y2 must be non-negative

	[[alternative HTML version deleted]]

