On Tue, Jun 19, 2012 at 8:22 AM, Gustavo Fernández Bayón
<gbayon@gmail.com>wrote:
>
> As I have said to Tim in a previous post, I really thought I did not have
> to deal with normalization issues. Now it seems I have to start worrying
> about it.


Maybe yes, maybe no.  Judgment matters when making such a decision.  In
some cases normalization seems to be a wash (see for example
http://genomebiology.com/2011/12/1/R10 ) whereas in others it appears to
make a material difference (
http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000952).
 My purpose in raising the point is just that investigators ought to
be
aware of the possibility for spurious differences, so that they are caught
early on rather than later.

Sorry if it sounded more alarmist than that.

--t

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