 Hi members,
I'm trying for quite some time to get an analysis started for affy
microarray files which has HuGene-1_0-st-v1.
justrma step worked.
> eset_justrma
ExpressionSet (storageMode: lockedEnvironment)
assayData: 32321 features, 18 samples
  element names: exprs, se.exprs
protocolData
  sampleNames: MC1_(HuGene-1_0-st-v1).CEL MC10_(HuGene-1_0-st-v1).CEL ...
MC9_(HuGene-1_0-st-v1).CEL (18 total)
  varLabels: ScanDate
  varMetadata: labelDescription
phenoData
  sampleNames: MC1_(HuGene-1_0-st-v1).CEL MC10_(HuGene-1_0-st-v1).CEL ...
MC9_(HuGene-1_0-st-v1).CEL (18 total)
  varLabels: sample
  varMetadata: labelDescription
featureData: none
experimentData: use 'experimentData(object)'
Annotation: hugene10stv1

But I am not able to do the annotation. I tried many possible options.
I keep getting this Error.
> biocLite("hugene10stv1.r3cdf")
Using R version 2.12.2, biocinstall version 2.7.7.
Installing Bioconductor version 2.7 packages:
[1] "hugene10stv1.r3cdf"
Please wait...

Warning message:
In getDependencies(pkgs, dependencies, available, lib) :
  package ‘hugene10stv1.r3cdf’ is not available

> library("annotate")
> library("hugene10stv1")
Error in library("hugene10stv1") :
  there is no package called 'hugene10stv1'

> library("hugene10stv1.db")
Error in library("hugene10stv1.db") :
  there is no package called 'hugene10stv1.db'

> biocLite("hugene10stv1.r3cdf", type = "source")
Using R version 2.12.2, biocinstall version 2.7.7.
Installing Bioconductor version 2.7 packages:
[1] "hugene10stv1.r3cdf"
Please wait...

Warning message:
In getDependencies(pkgs, dependencies, available, lib) :
  package ‘hugene10stv1.r3cdf’ is not available

Can anybody please help. Thanks a lot in advance.
Best wishes,
Suparna.

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