Dear Steve,

Thank you for your reply.  I have done microarray experiments with pooled
samples instead of replicates. I wanted to know if there is any technical
difficulty in AgiMicroRna if we have no replicates.

Thanks,

Karthik

On Thu, Jun 14, 2012 at 7:32 PM, Steve Lianoglou <
mailinglist.honeypot@gmail.com> wrote:

> Hi,
>
> On Thu, Jun 14, 2012 at 5:51 AM, Karthik K N <karthikuttan@gmail.com>
> wrote:
> > Dear Members,
> >
> > Do we need replicates to carry out analysis with AgiMicroRna package in
> > bioconductor? I have one control and one treated samples. Can I go ahead
> > ewith AgiMicroRna with these two datasets?
>
> It depends on what you mean by "need".
>
> I suspect that it is *technically* possible to carry out an analysis
> without replicates so that at the end of day you get *some* results.
> In that sense, you might not "need" replicates.
>
> If by "need" you mean that you want results that you can have some
> faith in, then yes: you need replicates ... always.
>
> -steve
>
> --
> Steve Lianoglou
> Graduate Student: Computational Systems Biology
>  | Memorial Sloan-Kettering Cancer Center
>  | Weill Medical College of Cornell University
> Contact Info: http://cbio.mskcc.org/~lianos/contact
>



-- 
Karthik K.N
Cancer Discovery Biology Laboratory
Division of Molecular Medicine
Amrita Center for Nanosciences and Molecular Medicine
Amrita Institute of Medical Sciences
AIMS-Ponekkara (P.O), Kochi
Cochin, Kerala - 682 041
+91-484-280 1234 x 8720
+91-9400193907

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