Dear users,

I have a question about miRNA differential profiling between two groups. Someone suggested a further filtering of other small RNAs such as mRNA degradation, etc with lengths similar to miRNA after BAM file (which pre-filtering out longer or shorter ones) read-in. I was wondering what consequences would be if I do NOT conduct this process. In this situation, a miRNA count table contains those contaminated non-coding small RNAs and those small RNAs are brought into DE analysis. My intuitive understanding is that it should be fine as long as the miRNA IDs (say, Entrez IDs)are not shared by other small RNAs during alignment or annotation.

thanks in advance.

sincerely,

William Wu

	[[alternative HTML version deleted]]

