Hi all,

I need to normalize GFP expression by cell size and look at the distributions for my data.  After I created the new variable, and tried to plot it using densityplot, I got a graph with the x and y axis but no plot.  After playing around with variations of the function, it seems as if it doesn't like dividing any number by one of the variables.  I'm not sure how to get it to work.  

Thanks,
Chandra

using the GvHD datase:
data(GvHD)
a_norm <- transform(GvHD, FL1norm = (`FL1-H`/`FSC-H`))
densityplot(~ FL1norm, a_norm[1]) # no plot



sessionInfo()
R version 2.15.0 (2012-03-30)
Platform: x86_64-apple-darwin9.8.0/x86_64 (64-bit)

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] psych_1.2.1         ggplot2_0.9.0       flowViz_1.20.0      lattice_0.20-6     
 [5] flowCore_1.22.0     rrcov_1.3-01        pcaPP_1.9-46        mvtnorm_0.9-9992   
 [9] robustbase_0.8-1-1  Biobase_2.16.0      BiocGenerics_0.2.0  BiocInstaller_1.4.4

loaded via a namespace (and not attached):
 [1] colorspace_1.1-1    dichromat_1.2-4     digest_0.5.2        feature_1.2.8      
 [5] graph_1.34.0        grid_2.15.0         KernSmooth_2.23-7   ks_1.8.7           
 [9] latticeExtra_0.6-19 MASS_7.3-18         memoise_0.1         munsell_0.3        
[13] plyr_1.7.1          proto_0.3-9.2       RColorBrewer_1.0-5  reshape2_1.2.1     
[17] scales_0.2.0        stats4_2.15.0       stringr_0.6         tools_2.15.0  






Chandra Jack
Postdoctoral Research Assoc.
Dept. of Bioengineering
Rice University
chanj@rice.edu




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