Hai,

I just want to know the model that we have to use for the LIMMA package for
analysing the agilent single colour data. Currently am using the model:

*design <- model.matrix(~ 0+factor(c(1,1,2,2)))*

*colnames(design) <- c("group1", "group2")*

*fit <- lmFit(y.ave, design)*

*print(design)

*
Is this model design suits for analyzing the Agilent single color data
using the LIMMA package without having any replicates? Instead of using the
replicate of the samples we have just used the pooled samples in analyzing
the data.


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