Hi All,

I used calcNormFactors function in edgeR to normalize my data :

Normalized->calcNormFactors(data)

However ,when i used it

Normalized1->calcNormFactors(data,refColumn=1)


I got slightly different normalization factors,The difference is about 0.02
between Normalized and Normalized1 for some libraries
For me it was not expected as i thought the function considers the 1st
column is the reference column by default.Any Comments??

Thank you and best regards
Chris

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