I'm not sure about the library size you mentioned.
> Is it the total reads of a sample or the sum of the reads mapped to each gene.
> I mean, if the total reads of a sample is 1,000,100,
> but when I mapped the reads to the gene, I got a list of gene with reads mapped to it, and the sum of this is 900,000.
> Do I use 1,000,000 or 900,000??

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