Hi all,
I met a question when I analysed mRNA data. I use TMM provided in edgeR to normalizition my data. After I got the factors, How Do I treat my raw counts?? In my oppion, (raw.counts)*10^7/(librarysize*factors),Is It right?
And another question is which librarysize do I need? The unique total reads or the reads mapped to each gene???
I need your help~~~
Best 

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