Dear Sir,

My name is Kannan. I have been trying to work on the image analysis of some
Agilent MicroRNA microarray data using R and Bioconductor. We have the
microarray data in the form of TIFF, JPEG images and Txt files. Having
installed R and also the relevant packages, we are not able to get on with
the Analysis properly. We were able to reach until reading the target
files, after which we face problem with readMicroRnaAFE. I have pasted
below the step that we got stranded in.Could you please offer some advice
and guide us in our work?


targets=readTargets(infile="targets.txt",verbose=TRUE)

Target File
  FileName Treatment GErep
1        1         C     1
3        3         T     2

> dd=readMicroRnaAFE(targets,verbose=TRUE)
Error in file(file, "r") : cannot open the connection
In addition: Warning message:
In file(file, "r") : cannot open file '1': No such file or directory
>


Thanks,
Kannan


-- 
Kannan Venugopal
MSc Biomedical Sciences
Bharathidasan University
Tiruchirappalli 620 024
Tamil Nadu
India
Contact No: 08870455429

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