Hi dear Fadi,

   The ddCt package currently only supports SDS format converted to TXT
file format. HTqPCR should do the job - we will consider implementing the
functionality in ddCt as well.

  Best wishes,
David

2011/12/1 Alnaji, Fadi <F.Alnaji@warwick.ac.uk>

> Deam Jim, thanks a lot.
>
> I'll try this out and let you know.
>
> All the best - Fadi
>
> -----Original Message-----
> From: jimrperkins@gmail.com [mailto:jimrperkins@gmail.com] On Behalf Of
> James Perkins
> Sent: 01 December 2011 11:42
> To: Alnaji, Fadi
> Cc: bioconductor@r-project.org
> Subject: Re: [BioC] ddCt isn't working with exported ABI7500-RT-PACR file
>
> Hey,
>
> You could try ReadqPCR or HTqPCR, both enable you to read in SDS files
> I believe (ReadqPCR has a read.qPCR function, and there is an
> explanation of how to do so in HTqPCR). If you were to have problems
> using your version of SDS file with ReadqPCR, I would be very grateful
> if you could email me your file (annonymsed if you wish of course) so
> I can alter ReadqPCR appropriately.
>
> Cheers,
>
> Jim
>
> On 1 December 2011 10:51, Alnaji, Fadi <F.Alnaji@warwick.ac.uk> wrote:
> >
> > Hello
> >
> > I am trying to use the ddCt package which was developed by Jitao David
> > Zhang, Rudolf Biczok and Markus Ruschhaupt. My problem is that it seems
> > the package is not able to read the exported file, the one contains the
> > individual ct Values, from ABI 7500 RT-PCR instrument. When I execute
> > the package, it says your file is not .SDM. That's true, as my file is
> > SDS, but this is the only possibility I have got in the machine. However
> > I tried to play a bit with the text format in excel to make SDS as
> > similar as the SDM in the package-example file. It worked indeed, but it
> > is not a practical way I believe.
> >
> > Is there any other option to make this package able to read my SDS
> > files, or to convert this file to SDM.
> >
> > Many thanks.
> >
> >
> >
> >
> >        [[alternative HTML version deleted]]
> >
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-- 
Jitao David Zhang
Computational Biologist

http://www.NextBioMotif.com/

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