did you try a google search with "bioconductor import GTF" -- there are a
few direct solutions including

http://manuals.bioinformatics.ucr.edu/home/ht-seq#TOC-Parsing-Gene-Intergenic-Regions-Bas


On Tue, Nov 29, 2011 at 2:05 PM, sunny [guest] <guest@bioconductor.org>wrote:

>
> Any R based package to integrate GTF format gene annotation to
> GenomicRanges object? or make a .sqlite object then can be saved
> (saveFeatures) for future use?
> really need this tool for RNAseq data analysis. Thank you very much!
>
>
>
>  -- output of sessionInfo():
>
> library(GenomicFeatures)
> library(Rsamtools)
>
> commands used: makeTranscriptsDbfromUCSC
>                           saveFeatures("ensGene.sqlite")
>                           loadFeatures("ensGene.sqlite")
>
>
> --
> Sent via the guest posting facility at bioconductor.org.
>
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