

Hi all, 
I am trying to load my agilent data to Limma using read.maimages function. Here's what I did: 
> library(limma)> targets<-readTargets("targets.txt")> RG <- read.maimages(targets, columns = list(G = "gMedianSignal", Gb = "gBGMedianSignal", R = "rMedianSignal",+    Rb = "rBGMedianSignal"), annotation = c("Row", "Col","FeatureNum", "ControlType","ProbeName"))

but I got the error message says: Read US83000170_252018610364_S01_GE2_105_Dec08_1_3.txt Read US83000170_252018610364_S01_GE2_105_Dec08_1_4.txt Read US83000170_252018610386_S01_GE2_105_Dec08_1_2.txt Read US83000170_252018610386_S01_GE2_105_Dec08_1_4.txt Error in readGenericHeader(fullname, columns = columns) :   Specified column headings not found in fileIn addition: Warning message:In file(file, "r") :  file("") only supports open = "w+" and open = "w+b": using the former

I already checked to made sure that all the columns I specified are existed in all my file. Does anyone know how to deal with this?
thanks a lot for your help
HC

 		 	   		  
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