Dear Bioconductor Members,

 I must start by saying that I'm just starting on analyzing the RNA-Seq data. I have RPKM values and their corresponding P-values for each gene.

I wanted to know if I'm correct using 
" N= RPKM x L x Ntot X 10-9
>where N = number of mapping reads at a given gene locus, L = estimated length (bp) of the gene locus, Ntot = number of total mapping reads, and RPKM = gene locus RPKM value "
to convert RPKM values into read counts. The read counts thus calculated using above formula can be used as input for DESeq? Do you suggest considering only statistically valid RPKM values (P-value < 0.05) for differential expression analysis using DESeq?


Thank you,
Avinash
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