Hi,
I have some microarrays to do analysis. It includes 2 factors: 1week old and 5week old as ageing factors (two level) , and 4_levels genotype (yw, GMH5xyw, GMH5xFOXO,GMH5x4EBP)
I assume the matrix is

1week 5week

yw b0 b0+b(Ageing)

GMH5xyw b0+b(Genetic)     b0+b(Ageing)+b(Genetic)

GMH5xFOXO    b0+b(Genetic)+b(FOXO)   b0+b(Genetic)+b(FOXO)+b(Ageing)

GMH5x4EBP   b0+b(Genetic)+b(4EBP)     b0+b(Genetic)+b(4EBP)+b(Ageing)




is it reasonable to do this analysis to extract effects of b(Genetic),b(Ageing),b(FOXO),b(4EBP) using limma and how to write matrix in code?

thanks



Zhi Zhang, Ph. D.
Sanford-Burnham Medical Research Institute
10901 North Torrey Pines Rd.
La Jolla, CA 92037, USA
Zhi Zhang, Ph. D.
Sanford-Burnham Medical Research Institute
Building 7, Bodmer lab
10901 North Torrey Pines Rd.
La Jolla, CA 92037, USA
Phone 858-646-3100 ext. 3801
Fax 858-795-5298



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