Hi,

I'd like to know if anyone has managed to use HTqPCR analysis for real time qPCR analysis, with deltaCt normalisation, but also incorporating a PCR efficiency correction? How would I go about doing this?

My second question concerns normalisation using deltaCt method. I have noticed that when there is a missing value as in Ct value missing, it fails to determine mean.  Not sure what to do here.

Thanks in advance.

Kind regards
Max

Maxy Mariasegaram| Reserach Fellow | Australian Prostate Cancer Research Centre| Level 1, Building 33 | Princess Alexandra Hospital | 199 Ipswich Road, Brisbane QLD 4102 Australia | t: 07 3176 3073| f: 07 3176 7440 | e: mariaseg@qut.edu.au


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