Dear Dr. Mattia Pelizzola：



I am sorry to trouble you!



I am great interested in the methods in your paper named Genome-wide screen
of promoter methylation identifies novel markers in melanoma.

I have downloaded this paper from the linking as fellow:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720187/



The methods of identification of biomarkers is very good. But I have
something wrong about it.



*Identification of biomarkers*

Intermediate and proximal promoter regions of each RefSeq were divided into
six regions based on the distance from the TSS (-500 to -350, -200 to -50, -50
to +100, +100 to +250, and +250 to +500 bp, with respect to the TSS). The
AMS for a given region was determined as the average of the AMS for the
probes in that genomic area.



I don't know you according to what rules to divide the RefSeq.Can you give
me an example to descipe it?



I will appreciate any help from you, thank you very much !

Best wishes

Yours sincerely

                  shujiema

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