I am currently using DESeq_1.5.23 and need to identify DEGs using RNA-Seq
read count data.
As  I hv no replicates wid me I had to go wid estimateDispersions command
wid method="blind",
and got very few DEGs (~10 only) along wid d warning msg to use
"sharingMode=fit-only " using which I am gettin ~1250 DEGs.

On performing the d same experiment wid older DESeq_1.5.1, I am getting
~2050 DEGs. I am confused on which result I should rely upon???????
Can u please suggest me which result is more significant?????????

-- 
Pushp Priya
NIPGR, New Delhi
India

	[[alternative HTML version deleted]]

