Hi Sean,

I used following commands to load the files in R. I created a target.txt
file with content as
SerialNumber FileName Condition
1 Normal1.txt Normal

>library(limma)
>target<-readTargets()
>RG<-read.maimages(target,(targets,columns = list(G = "gMedianSignal", Gb =
"gBGMedianSignal", R = "gProcessedSignal",Rb = "gIsPosAndSignif"),
annotation = c("Row", "Col","FeatureNum", "ControlType","ProbeName")))
I used some values of R and Rb

After it reads first 18 files it throws the message:

Read Normal18.txt
Read Wles1.txt
Read Wles2.txt
Error in RG[[a]][, i] <- obj[, columns[[a]]] :
  number of items to replace is not a multiple of replacement length



The sessionInfo() gives:
> sessionInfo()
R version 2.11.1 (2010-05-31)
i386-pc-mingw32

locale:
[1] LC_COLLATE=English_United States.1252
[2] LC_CTYPE=English_United States.1252
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C
[5] LC_TIME=English_United States.1252

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
[1] limma_3.4.5

loaded via a namespace (and not attached):
[1] Biobase_2.8.0


Please help.

Regards,
Srikanth

On Wed, Aug 3, 2011 at 6:22 PM, Sean Davis <sdavis2@mail.nih.gov> wrote:

> Hi, Srikanth.
>
> Please always provide the code you are using (cut-and-paste), the
> error message, and sessionInfo() when asking for help.  That will help
> the list members to more easily figure out what the problem might be.
>
> Sean
>
>
> On Wed, Aug 3, 2011 at 8:49 AM, Srinivas M. Srikanth
> <srikanth@ibioinformatics.org> wrote:
> > Hi all,
> >
> > I have data from single color agilent array exposed and unexposed to
> drug.
> > The exposed data is from 1x44 platform and unexposed from 4x44k. When i
> try
> > limma and load the files using read.maimages i get the error:
> >
> > Error in RG[[a]][, i] <- obj[, columns[[a]]] :
> >  number of items to replace is not a multiple of replacement length
> >
> > Please help me with this. How can load and perform analysis on this data.
> >
> > Regards,
> > Srikanth
> >
> > --
> > Srinivas M. Srikanth
> > Ph.D. Student
> > Institute of Bioinformatics
> > Discoverer, 7th Floor,
> > International Technology Park,
> > Bangalore, India
> > Mob:+917259692031
> >
> >        [[alternative HTML version deleted]]
> >
> > _______________________________________________
> > Bioconductor mailing list
> > Bioconductor@r-project.org
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> > Search the archives:
> http://news.gmane.org/gmane.science.biology.informatics.conductor
> >
>



-- 
Srinivas M. Srikanth
Ph.D. Student
Institute of Bioinformatics
Discoverer, 7th Floor,
International Technology Park,
Bangalore, India
Mob:+917259692031

	[[alternative HTML version deleted]]

