Hello all,

I would like to create a linear model where i have 4 tissues, 2 types of
mice and 3 time points.
I created the following data frame:

Type    Tissue   Time  Value

Ty1      Tis1       T1     V1
Ty2      Tis1       T1     V2
...

For each Type Tissue and Time point i have 3 replicates.
I need to create a model like Y ~ Type x Tissue x Time and calculate the
contrasts (all possible combinations).
I need some help on the way i can use limma to do something like the above.

For example something like:
design = model.matrix (~ Data$Type x Data$Tissue x Data$Time)
would it work?
How can i take all the possible contrasts?


thanks in advance,
Dimitris

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