
Dear list

Using the qpcrNorm package I can read single data files into R with readQpcr() but when I try to read in multiple files with readQpcrBatch() I'm getting an error and I don't understand what it's trying to tell me. Please see below.

Many thanks
John


[john@y90-w-d RAT]$ R --vanilla

R version 2.13.0 (2011-04-13)
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ISBN 3-900051-07-0
Platform: i386-redhat-linux-gnu (32-bit)

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> library(qpcrNorm)
Loading required package: Biobase

Welcome to Bioconductor

  Vignettes contain introductory material. To view, type
  'openVignette()'. To cite Bioconductor, see
  'citation("Biobase")' and for packages 'citation(pkgname)'.

Loading required package: limma
Loading required package: affy
> x<-readQpcr("RAT_CT2.csv")
> slotNames(x)
[1] "geneNames"  "plateIndex" "exprs"      "normalized" "normGenes" 
> rm(x)



> x<-readQpcrBatch()
Error in initialize(value, ...) : 
  no slot of name "primerNames" for this object of class "qpcrBatch"



> sessionInfo()
R version 2.13.0 (2011-04-13)
Platform: i386-redhat-linux-gnu (32-bit)

locale:
 [1] LC_CTYPE=en_GB.utf8       LC_NUMERIC=C             
 [3] LC_TIME=en_GB.utf8        LC_COLLATE=en_GB.utf8    
 [5] LC_MONETARY=C             LC_MESSAGES=en_GB.utf8   
 [7] LC_PAPER=en_GB.utf8       LC_NAME=C                
 [9] LC_ADDRESS=C              LC_TELEPHONE=C           
[11] LC_MEASUREMENT=en_GB.utf8 LC_IDENTIFICATION=C      

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] qpcrNorm_1.8.0 affy_1.28.1    limma_3.6.9    Biobase_2.10.0

loaded via a namespace (and not attached):
[1] affyio_1.18.0         preprocessCore_1.12.0
> traceback()
4: initialize(value, ...)
3: initialize(value, ...)
2: new("qpcrBatch", geneNames = x@primerNames, plateIndex = x@plateIndex, 
       exprs = x.data, normalized = FALSE, normGenes = x@normGenes)
1: readQpcrBatch()

 		 	   		  
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