Hi All,

I am trying to represent my data as a heatmap without scaling it, in such a
way that the genes that are upregulated (respect to the control) are "green"
and those that are downregulated (respect to the control) are "red".

In order to do this, I normalized the data respect to the control, now the
upregulated genes have a value >1, and the downregulated genes have a value
<1. However, if I do a simple heatmap.2 (defining: scale = "none"), most of
the genes show the same color, and this is because the data is not centered,
and its distribution is skewed to the right.

But I would like to capture the differences on gene expression without
removing the higher values, and without centering the data. So, my guess is
that I have to modify the color key, but after trying different things and
couldn't find a way to do it.

Could you please give me some clues?

I appreciate any help!

Avhena

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