Dear Gordon,

Thanks, I followed that already. My question is how can i treat each of my
family samples as technical replicates and different family as a biological
replicate. And if that is what you would suggest.

Best,

Luis Zapata

On Wed, Mar 16, 2011 at 7:39 PM, Gordon K Smyth <smyth@wehi.edu.au> wrote:

> Dear Luis,
>
> Are you asking how to construct a design matrix?  Why not
>
>  design <- modelMatrix(targets, ref="C")
>
> as described in the limma User's Guide?
>
> Best wishes
> Gordon
>
>  Date: Tue, 15 Mar 2011 14:52:05 -0400
>> From: Luis Z <luisgls@gmail.com>
>> To: bioconductor@r-project.org
>> Subject: [BioC] limma design experiment
>> Message-ID:
>>
>> Hi everyone,
>>
>> I would like to receive a suggestion on the design model for this
>> experiment.
>>
>> There are 5 different RNA pool families. Each one with three replicates
>> (Technical?), coming from individuals that receive three different
>> treatments, a control treatment and T1, T2. The experiment were made :
>>                 fam    Cy3   Cy5
>> slide 1         F1    T1   C
>>                 F1    T1   C
>>                 ....
>>                 F5     T1   C
>>                 F1     T2   C
>>                 ...
>>                 F5      T2   C
>>
>> What would you suggest?
>>
>> Thanks,
>>
>> Luis
>> --
>> Luis Zapata Ortiz
>> Molecular Biotechnology Engineering
>> Laboratory of Bioinformatics and Mathematics of the Genome, LBMG.
>> Center of Mathematical Modeling of the University of Chile.
>> Av. Blanco Encalada 2120
>>  <56-2-9784551>56-2-9784551
>>
>
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