Dear Bioconductor users,

We have a univariate readout (DNA content) to study cell cycle
subpopulations. The data looks like this, with around 3000 cells per sample.


                cell1  cell2 cell3 ...
sample1    28     26    30
sample2    25     27    15
sample3    30     40    45
...


Based on which, one should be able to calculate fractions of cell cycle
subpopulations (G1, S, G2+M). However, the data needs to be first normalized
(scaling and peak alignment etc), before gating the cells into
subpopulations.

The flowCore and related packages offer similar functions, but seem to be an
overkill for a univariate readout. I wonder if there are other
methods/packages available.

Thanks a lot in advance!

Xian

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