Hello: 

I have faced a problem when I´m trying to analyze my TLDA data:

I have 3 or 2 technical replicates per sample, my problem is that I really don´t know how to read and asses the replicates. 
What I tried is to analyze my data as individual samples and then asses the replicates as  following:

d.normA2= exprs(d.normA)
> d.normA2.prom= (d.normA2[,1]+ d.normA[,2]+ d.normA2[,3])/3)
> d.normA2.prom= cbind(d.normA2.prom,(d.normA2[,4]
+ d.normA2[,5]+ d.normA2[,6])/3)
> d.normA2.prom= cbind(d.normA2.prom,(d.normA2[,11]
+ d.normA2[,12]) + d.normaA2[,13])/3)

But I get: Error: inesperado ')' en "d.normA2.prom= (d.normA2[,1]+ d.normA[,2]+ d.normA2[,3])/3)"

I don´t know which is the best option to read my replicates because I´m trying to evaluate 74 TLDA files corresponding to 30 samples, with technical replicates.

Thank you a lot.

Biol. Sandra Lorena Romero Córdoba
Ph student at Autonomous National University of Mexico
Lab. Genomics of Cancer, National Institute of Genomic Medicine
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