Hi Group,
Let me clearly explain.I have the [Mouse430_2] Affymetrix Mouse Genome 430
2.0 Array.I want to create an unnormalised expression microarray data set.I
have the cell files and cdf file for this.I want the intensities in the
probe level.Is this possible in R or any other source? or how can I get this
expression microarray dataset?
Thank you in advance,
Viritha

On Wed, Dec 15, 2010 at 4:05 PM, viritha kaza <viritha.k@gmail.com> wrote:

> Hi group,
> If I want to create raw txt file of microarray data from the (affymetrix)
> cell file, how do I create the expression set with raw signal intensity.I
> know that only cell file with the version 3 can be opened as excel file as
> it is in ascii format.
> In one such cell file the intensity is indicated as:
>    CellHeader=X Y MEAN STDV NPIXELS 0 0 137.3 25.1 36 1 0 10730.5 2009.9
> 36 2 0 136.3 21.2 36
>         But I am not sure how to assign the probe numbers to the
> CellHeaders and I would also like to know if the raw intensity taken is just
> the mean intensity? Can this be performed in R?
> Waiting for your response,
> Thank you in advance,
> Viritha
>

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