Hi ,

I have RNA-seq count data for 30,400 genes across 6 conditions (3 replicates
per condition). I was trying different normalization methods and then test
for differentially expressed genes between conditions. How to test whether
estimateSizeFactors() and estimateVarianceFunctions() does a good fit for my
data? Also is there a way to test whether the normalization is good ? Any
help is greatly appreciated,

Thanks,
Shrey

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