Dear List,

I am having some problems running oneChannelGUI to analyze some HuGene 1.0
ST arrays.

I have installed all the necessary components according to the installation
instructions. The program opens fine, I am able to select my working
diretory and my RNA target file. The problem occurs when I try to normalize
my data. I keep getting the following error message:


Gene level probe sets summary started

FATAL ERROR: .\TsvFile\TsvFile.cpp:2482: open: Could not open file:
'C:/Users/mbpinho/Documents/Microarray' to read.

Gene level probe sets summary ended
Warning messages:
1: In file(file, "rt") : only first element of 'description' argument used
2: In file(file, "rt") :
  cannot open file 'C:/Users/mbpinho/Documents/Microarray DATA/INCA
Data/Uniscience/out6db54a37/': No such file or directory
>

This problem only occurs when I am trying to analize the new Hugene 1.0 ST
arrays, if instead I use other data with 3' IVT arrays, the software runs
fine. I have already installed the APT tools from Affymetrix as well as the
Tcl/Tk extensions.

Any suggestions on how to fix my problem??

Regards,
-- 
Marcos B. Pinho
Programa de Engenharia Química - PEQ
Laboratório de Engenharia de Cultivos Celulares- LECC
Universidade Federal do Rio de Janeiro - UFRJ
Instituto Nacional de Câncer - INCA
Rio de Janeiro - Brasil

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