Hi all,

I would like to ask your expert opinions related to microarray experiments
with Drosophilas.


I have been asked to conduct a microarray experiment. According to the study
design (pilot study) I need 2-4 groups : 1st->3rd treatments + 4th control.
For each group 8-10animals would be ideal for the statistical power we want
to achieve. (4 array groups - 8 arrays per group)

If the experiment would be based on mice all that would be well as we need
the brain nerve tissue, and we can get enough quantity of total RNA from
mice brain. So one mice head goes into one microarray.

But what happens with Drosophilas? Do you mix lets say 100 heads to get
enough RNA? I believe pooling RNA  would create technical replicates with no
particular statistical value, and especially without a way to ascertain the
biological validity of results.(100 mixed heads per 1 microarray, 8
microarrays per group= no clear way to understand which important gene is
really overexpressed)

I welcome your expert opinions.

Kind regards,
-- 
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    Triantafyllos Paparountas
    - PhD+MSc Bioinformatics
    - BSc Molecular Medicine & Biochemistry
    - MSc Med. Statistics (Underway)
  http://www.bioinformatics.gr
  email: t_paparountas@bioinformatics.gr
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     Affymetrix Microarray Analysis Expert
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