qPCR workshops
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Life Science is still a growing sector and new methods and technologies are continously developed. Therefore permanent training and education becomes so important.
With our specific course program we are offering a range of high-quality course modules to give a general and independent overview of existing qPCR technologies and systems. Our course issues are based on skilled know-how from own research studies and publications. Our aim is to point out a critical way of thinking to increase the quality and outcome of experimental data.

All BioEPS workshops are based on the MIQE guidelines (Minimum Information for Publication of Quantitative real-time PCR Experiments), the first guidelines for quality assurance within qPCR technology => MIQE.gene-quantification.info http://miqe.gene-quantification.info/


FLYER => http://www.gene-quantification.de/bioeps-flyer-2010.pdf


All courses are held regularly in Freising-Weihenstephan, Germany, in German and English language. Further customized workshops and specialized trainings will be held as well across Europe and world-wide. Workshops are powered by BioEPS GmbH http://www.bioeps.com/, located at the campus of the Technical University of Munich, in Freising-Weihenstephan, very close to the Munich Airport (MUC).
For more information and registration, please see our web page => http://workshops.gene-quantification.info/

New qPCR Basis Workshop Module from 14 - 16th July 2010

The introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision.

About the tutors:
Dr. Michael Pfaffl is one of the leading experts in qRT-PCR and in qPCR data analysis, specialiced on relative quantification strategies. He is author of many highly influental publications.
Dr. Martina Reiter is qPCR specialist with focus on experiment setup and many years of experience in the optimization of qPCR experiments on vrious qPCR platforms.



Register here => http://site.bioeps.com/index.php?option=com_seminar&Itemid=6


Day 1:
    * Basic PCR theory and applications
    * Detection Chemistry (SYBR Green I, TaqMan, Molecular Beacons...etc)
    * Different instrument platforms and their typical uses
    * Primer Design, Probe design and optimization
    * Basic data handling and analysis

Day 2:
     * Experimental setup and how it influences qPCR data
     * Nucleic acid extraction
     * cDNA synthesis and pre-amplification
     * Introduction to specific qPCR applications: genotyping, SNP analysis, HRM

Day 3:
    * Introduction to quantification principles
    * Quantification strategies and qPCR efficiency
    * Absolute quantification
    * Relative quantification
    * Strategies for normalization of qPCR data

HRM Module:
The introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains:
   * Introduction to HRM technology
   * Experimental setup
   * SNP-analysis
   * Gene scanning
   * Methylation analysis
   * Kits and instruments
   * Applications


Course modules:
* 3-day qPCR Basic Module
* 2-day Experiment Design & qPCR data processing NEW !
* 3-day single-cell qPCR
* 2-day microRNA qPCR
* 1-day HRM
* 2-das qPCR-R data analysis NEW !
* 1-day Project Management NEW !
* 2-day Quality Management NEW !



Course dates 2010:
* 12 - 14 July 2010 (E) 3-day qPCR Basic Module (Mon. - Wed.)
* 4 - 6 October 2010 (E) 3-day qPCR Basic Module (Mon. - Wed.)
* 7 October 2010 (E) 1-day HRM Module (Thu.)
* 11 - 12 October 2010 (E) 2-day qPC-R - data analysis using R packages (Mon.-Tue.)
* 18 - 20 October 2010 (E) 3-day single-cell & qPCR (Mon. - Wed.)
* 21 - 22 October 2010 (E) 2-day Experiment Design & qPCR data processing (Thu. - Fri.)
* 8 - 9 November 2010 (E) 2-day microRNA & qPCR (Mon.-Tue.)
* 29 November - 1 December 2010 (E) 3-day qPCR Basic Module (Mon. - Wed.)
* 2 - 3 December 2010 (E) 2-day Experiment Design & qPCR data processing (Thu. - Fri.)
Download course brochure 2010 http://www.gene-quantification.de/bioeps-course-programm-2010.pdf

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In our workshop GenEx software will be used for data analysis
http://genex.gene-quantification.info/


GenEx offers advanced methods to analyze real-time qPCR data with simple clicks of the mouse.
GenEx is the leading software for processing and analysis of qPCR data. It provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to cutting-edge data analysis.

Near universal instrument compatibility
GenEx is already compatible with instruments from most of the main qPCR instrument producers and the list of compatible instruments keeps growing with the help and input from existing customers.

User-friendly interface
GenEx has a user friendly interface designed to be both easy to learn for a novice and flexible enough for the demands of a professional user.

Comprehensive pre-processing and data editing
Possibly the most important part of qPCR experiments is to pre-process the raw data for subsequent statistical analyses. The pre-processing steps need to be performed consistently in correct order and with confidence. GenEx contains comprehensive tools for data pre-processing and data editing.

Cutting-edge data analysis
GenEx offers cutting-edge data analysis tools, with GenEx Pro and Enterprise being the most complete versions. Current features include parametric and non-parametric statistical tests, clustering methods, principal component analysis, artificial neural networks, and much more. New features are continuously being added to GenEx with close attention to customer needs. In the GenEx forum you will find in depth information about experimental design, data analysis and GenEx features.

http://genex.gene-quantification.info/


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