Dear all,

I have a data set that consists of 36 qPCR plates:
3- cond1
2-cond 2
1-cond3

when I make the normalization using the endogenous gene I get the following
message:

Calculating deltaCt values

> >         Using control gene(s): U6
> >         Card 1: Mean=33.62      Stdev=NA
> >         Card 2: Mean=32.65      Stdev=NA

So after seing the normalized data, I saw that the normalization is just
making the difference between the Ct of the endogenous gene and the Ct of
the target gene. Shouldn't the normalization take into account the variance
of the Ct value of the endogenous gene across all samples and conditions in
order to normalize and create comparable expression values between
conditions?
With kind regrads,
Andreia

-- 
--------------------------------------------
Andreia J. Amaral
Unidade de Imunologia Clínica
Instituto de Medicina Molecular
Universidade de Lisboa
email: andreiaamaral@fm.ul.pt
          andreia.fonseca@gmail.com

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