Hi
I am working on two color miRNA exiqon data, I could able to normalize data
but the problem is once data is normalized  my list contain 4 "within-array
replicates" so I want make it to single miRNA's
I tried to follow this thread "Read single channel GenePix in limma " by
Gordon which works good for single channel data but when it come for two
channel I am getting following error.


Sequences of steps i am doing

RG <- read.maimages(files, targets$FileName,columns=list(R="F635
Mean",G="F532 Mean",Rb="B635 Mean",Gb="B532 Mean"))
dat <-normalizeBetweenArrays(RG, method="quantile")
> dat2 <- log (dat)
*Error in log(dat) : Non-numeric argument to mathematical function

*rownames(dat@hx) <- RG.final$genes$Name*
Error in rownames(dat@hx) <- RG.final$genes$Name :       ---* this is
basically where one can insert rownames into matrix with normalized data
*  no slot of name "hx" for this object of class "MAList"


Any help is greatly appreciated

Thanks
Ashwin



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