Hello Josef,

Thank you very much for the information!
Following your advice, I tried to use the flowCore package, and I was able
to load one of the files I have. It was loaded without any problems.
Following is the output:

library(flowCore)
x <- read.FCS('path to LMD file')

flowFrame object 'A0-5  015.LMD'
with 35117 cells and 6 observables:
       name    desc range minRange maxRange
$P1  FS LIN  FS LIN  1024 0.000000     1023
$P2  SS LIN  SS LIN  1024 0.000000     1023
$P3 FL3 LIN  PI LIN  1024 0.000000     1023
$P4     AUX PI PEAK  1024 0.000000     1023
$P5 FL3 LOG  PI LOG  1024 1.000922    10000
$P6    TIME    TIME  1024 0.000000     1023
139 keywords are stored in the 'descripton' slot
> summary(x)
        FS LIN SS LIN FL3 LIN  AUX FL3 LOG   TIME
Min.       4.0    1.0    25.0   23   244.9   0.00
1st Qu.  233.0  145.0   392.0  343  3851.0  22.00
Median   333.0  222.0   495.0  404  4866.0  52.00
Mean     402.6  284.8   584.1  449  5725.0  56.81
3rd Qu.  509.0  352.0   795.0  531  7772.0  89.00
Max.    1023.0 1023.0  1023.0 1023 10000.0 134.00

However, when I tried to visualize the data with the flowViz package, after
typing:
library(flowViz)
plot(x)

I get the following error:
Error in x@parameters$name :
  '.SigArgs' is shorter than '.SigLength' says it should be

Actually, I am interested in analyzing cell cycle data, and I thought that I
would be able to do that with the iFlow or flowCore + flowViz packages.
Is there another option that can be helpful for that?

Thank you in advance!

Regards,
Anna

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