Dear Christian,

thank you for your help. I will have a look at your package for sure. Actually, we already have it installed on our servers (along with root), and will try it with the 6 sample CEL files provided by Affy's website (I'm still waiting for our own data).

Thank you so much!

Xavi.


-----Mensaje original-----
De: cstrato [mailto:cstrato@aon.at]
Enviado el: lun 28/06/2010 20:11
Para: Sole Acha, Xavi
CC: James W. MacDonald; bioconductor@stat.math.ethz.ch
Asunto: Re: [BioC] Affymetrix HG U295 support in BioC?
 
Dear Xavier,

Alternatively, you could use package xps, which also supports the 
HG-U219 arrays. It uses the official CDF and annotation files from 
Affymetrix to create so-called "root scheme files". However, you can 
download the pre-built scheme file "hgu219_na30.root" from:
/

Besides rma normalization xps also allows you to do mas5 normalization 
for PM-only arrays.

Best regards
Christian
_._._._._._._._._._._._._._._._._._
C.h.r.i.s.t.i.a.n   S.t.r.a.t.o.w.a
V.i.e.n.n.a           A.u.s.t.r.i.a
e.m.a.i.l:        cstrato at aon.at
_._._._._._._._._._._._._._._._._._


On 6/28/10 7:19 PM, Sole Acha, Xavi wrote:
> Hi Jim,
>
> Sorry... that was a mistake of mine. Certainly I was talking about U219 arrays.
>
> Are the cdf files you've built available? That would be of great help to me.
>
> Besides that... I guess there is a kind of mas5-like normalization for PM-only arrays... maybe newer versions of BioC may handle it.
>
> Thank you very much for your help.
>
> Xavi.
>
>
>
> -----Mensaje original-----
> De: James W. MacDonald [mailto:jmacdon@med.umich.edu]
> Enviado el: lunes, 28 de junio de 2010 18:18
> Para: Sole Acha, Xavi
> CC: bioconductor@stat.math.ethz.ch
> Asunto: Re: [BioC] Affymetrix HG U295 support in BioC?
>
> Hi Xavier,
>
> Sole Acha, Xavi wrote:
>> Dear listers,
>>
>> I would be pleased if someone could tell me if there are any plans to
>> include the new Affymetrix HG U295 arrays in the list of supported
>> platforms for "standard" affy analysis packages (affy,
>> simpleaffy,...). Although the array came out a few months ago, it
>> seems that the CDF has not been included in the BioC release yet. By
>> now, the only BioC package I've found that seems to support these new
>> array plates is xps. Does someone know anything more about this?
>
> I don't see a HG-U295 chip on the Affy website, but I do see a HG-U219.
> Is that a typo on your part?
>
> If not, can you give a link to the correct webpage?
>
> I have built cdf and probe packages for the HG-U219 chip, but note that
> these will only work with the affy and affyPLM packages. Since the chips
> are PM-only, they won't work with simpleaffy, and will require some work
> on your part to get to work with gcrma, if at all.
>
> Best,
>
> Jim
>
>
>>
>> Thank you very much for your help in advance.
>>
>> Best regards,
>>
>> ------ Xavier Solé Acha Unitat de Biomarcadors i Susceptibilitat Unit
>> of Biomarkers and Susceptibility Institut Català d'Oncologia //
>> Catalan Institute of Oncology Gran Via de L'Hospitalet 199-203 08907
>> L'Hospitalet de Llobregat, Barcelona, Spain. Phone: +34 93 260 71 86
>> / +34 93 335 90 11 (ext. 3194) Fax: +34 93 260 71 88 E-mail: x.sole
>> (at) iconcologia.net
>>
>> _______________________________________________ Bioconductor mailing
>> list Bioconductor@stat.math.ethz.ch
>> https://stat.ethz.ch/mailman/listinfo/bioconductor Search the
>> archives:
>> http://news.gmane.org/gmane.science.biology.informatics.conductor
>



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