sir,
      puma clustering method . i make the object of (sample6, Celfile)  by
this command

dat<-ReadAffy()

library(pumadata)

>eset_mmgmos<-mmgmos(dat)
>eset_rma<-rma(dat)

after that i do "dentifying differentially expressed genes" method in this
method i use this command
>eset_comb <- pumaComb(eset_mmgmos)
> pumaDERes <- pumaDE(eset_comb)
> limmaRes <- calculateLimma(eset_rma)      ## in this command it show the
 error like:
Error in ebayes(fit = fit, proportion = proportion, stdev.coef.lim =
stdev.coef.lim) :
  No residual degrees of freedom in linear model fits
i don't understand it
plz solve this and mail me .

i more things
in the code
toppumaDEIntGene<-topGenes(pumaDERes, contrast = 7)

i don't under stand the value of contrast, how it's value define ,in my obj
have 6 cel file ,so plz help me to define its value.

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