List,
       sir , i have create the object by  downloading CEL file through Array
Express,now I want to do puma clustering method, for this i use "*puma: a
Bioconductor package for propagating uncertainty in *
*microarray analysis*" for guideline, in this method i done pumaPCA
method,but in the second step "Identifying differentially expressed genes"
show error  in this command
limmaRes <- calculateLimma(eset_estrogen_rma)
show error:
limmaRes <- calculateLimma(eset_rma)
Error in ebayes(fit = fit, proportion = proportion, stdev.coef.lim =
stdev.coef.lim) :
  No residual degrees of freedom in linear model fits

i don't understand it .Plz mail me any help or suggestion about it .

Regards
avinash

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