



Hello Pedro,

I am using AgiMicroRna
1.0.0 to process my agilent miRNA microarray data. Agilent Feature Extraction
Software v10.7 was used to extract the features. I would like to use readMicroRnaAFE
function to read in the extracted feature data files. However, the error
message says:

Error in
readGenericHeader(fullname, columns = columns, sep = sep) : 

  Specified column headings not found in file


I check the feature
data files, there are several relevant columns missing: gMeanSignal, gBGUsed, chr_coord.
But I still want to use readMicroRnaAFE to create RGList so that AgiMicroRna can be used for further QC and
analysis. Is it possible to do some small modification of the readMicroRnaAFE
function to read in the data and still able to use the downstream utility (QC,
RMA cross-array normalizationm, miRBase annotation linking etc) in AgiMicroRna?
Thanks a lot for your input and help!

Heyi




      
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