I am afraid I do not know. I am following the instructions as set on
Flow cytometry data analysis using Bioconductor - A typical work flow.

Nishant Gopalakrishnan

July 23, 2009

 

And I can't load the fcs files.

 

-----Original Message-----
From: Chao-Jen Wong [mailto:cwon2@fhcrc.org] 
Sent: Wednesday, February 17, 2010 6:04 PM
To: Sancho, Jose
Cc: bioconductor@stat.math.ethz.ch
Subject: Re: [BioC] Problem reading files on FlowCore

 

Hi Jose,

 

May I ask why there is a "+" sign before the transformation argument?

 

-C.J.

 

Sancho, Jose wrote:

> I am new to FlowCore, and I am trying to get the demo files,as
described

> on Flow Cytometry data analysis using Bioconductor- A typical work
flow,

> and having difficulties. 

> 

>  

> 

> I type the instruction flowData <-read.flowSet(path = "C:\Documents
and

> Settings\sanjo05\My Documents\BIOCONDUCTOR SOFTWARE", phenodata =

> "annotation.txt", + transformation = FALSE).

> 

>  

> 

> I get the following error message Error: unexpected '=' in "flowData

> <-read.flowSet(path = "C:\Documents and Settings\sanjo05\My

> Documents\BIOCONDUCTOR SOFTWARE", "annotation.txt", + transformation
="

> 

>  

> 

> What am I doing wrong?

> 

>  

> 

> Thanks

> 

>  

> 

> Jose Sancho

> 

>  

> 

> Genzyme Corporation

> 

> Flow Cytometry Core Facility

> 

> 

>     [[alternative HTML version deleted]]

> 

> _______________________________________________

> Bioconductor mailing list

> Bioconductor@stat.math.ethz.ch

> https://stat.ethz.ch/mailman/listinfo/bioconductor

> Search the archives:
http://news.gmane.org/gmane.science.biology.informatics.conductor

>   

 

 

-- 

Chao-Jen Wong

Program in Computational Biology

Division of Public Health Sciences

Fred Hutchinson Cancer Research Center

1100 Fairview Avenue N., M2-B876

PO Box 19024

Seattle, WA 98109

206.667.4485

cwon2@fhcrc.org

 


	[[alternative HTML version deleted]]

